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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in lots of tumors, which results in chromosome instability, and thus could lead to tumorigenesis. This protein was identified to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a critical purpose in marketing mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually described. Two added splice variants were being claimed but detailed nucleotide sequence details isn't yet obtainable.
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Blocking peptides are peptides that bind specially on the goal antibody and block antibody binding. These peptide usually includes the epitope acknowledged from the antibody.
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This information outlines additional probable optimization of mobile and immunoassay parameters to guarantee the best possible success are...
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This guideline outlines even more possible optimization of cellular and immunoassay parameters to be certain the best possible effects are...
Antibodies bound to the blocking peptide not bind on the epitope to the focus on protein. By evaluating the staining within the blocked antibody vs . the antibody alone, you can see which staining is unique.
Target Data Cyclin E belongs for the remarkably conserved cyclin relatives, whose customers are characterized by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins function as regulators of CDK kinases. Different cyclins show distinctive expression and degradation designs which lead towards the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and functions to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is also degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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